Objective To construct a melanoma cell strain expressing endotopic CSDE1-EGFP using new CRISPR-PITCH system, then to select the cell strain of low expression CSDE1 and high expression CSDE1.Methods The EGFP coding sequence was inserted into the last exon of the CSDE1 gene using a micro-homology-mediated end joining(MMEJ) method; Using flow cytometr
In view of the fact that Coffee Machine Descaler the existing defog methods often ignore the key atmospheric light estimation, a method based on quadtree decomposition is proposed, which avoids the influence of bright white area on atmospheric light estimation and accurately estimates atmospheric light in the sky region.In order to avoid the limita
Coronaviruses of potential recombinant origin with porcine transmissible gastroenteritis virus (TGEV), referred to as a new subtype (IIb) of canine coronavirus (CCoV), were recently identified in dogs in Europe.To assess the distribution of the TGEV-like CCoV Coal copyright subtype, during 2001–2008 we tested fecal samples from dogs with gastroen
The Magnetic Pulse Welding (MPW) process uses only electromagnetic force to create a solid-state metallurgical bond between a working coil and outer workpiece.The electromagnetic force drives Book the outer tube to collide with the inner rod, resulting in successful bonding.However, due to the dissimilarity of the MPW joint, only a portion of the i
On the basis of economic and institutional analysis of economic activity and the theory of intrinsic motivation proposed general scheme of individual motivation of economic activities, with the establishment of the major economic and psychological factors in its Urn Element formation.In accordance with the proposed scheme analyzes the main motivati